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Part:BBa_K145104:Design

Designed by: Jonas Demeulemeester   Group: iGEM08_KULeuven   (2008-07-10)


T7 PoPS + RiboLock -> LacI -> inhibition of LuxI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1250
    Illegal BglII site found at 2120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Check F1610. See whether the Lac promotor is strong enough! Modulate with IPTG? Strong RBS is in front of the LuxI (efficiency 1)


Source

other parts

References